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Image Search Results
Journal: JCI insight
Article Title: Metabolism of PLTP, CETP, and LCAT on multiple HDL sizes using the Orbitrap Fusion Lumos.
doi: 10.1172/jci.insight.143526
Figure Lengend Snippet: Figure 2. An interinstrument platform comparison of PLTP and CETP tracer enrichment data. (A) PRM scans of the same CETP peptide from the Q Exactive and the Lumos. The relative peak intensities of the fragment ions are conserved between the 2 instruments. The y7 2HM3 (tracer) peak environment is zoomed in. R = 120 K for the Q Exactive and 240 K for the Lumos. (B) Loess regression plots showing that the standard error (gray) of the fitted curves is lower on the Lumos. PLTP data are from alpha1 and CETP from alpha2. Legend: the PRM ions’ m/z values. (C) Box plots depicting the distribution of the enrichment data in B. Each data point is the average enrichment (n = 3 PRM ions’ measurements) per time point. The box plots depict the minimum and maximum values (whiskers), the upper and lower quartiles, and the median. The length of the box represents the interquartile range. The gray lines indicate the relative shift in enrichment value per given time point. Variance was calculated using individual PRM ion enrichment data in B, not the averages.
Article Snippet: Each protein was quantified using the following peptide standards: APOA1 (THLAPYSDEL[R-labeled]), APOE (LGPLVEQG[R-labeled]), LCAT (SSGLVSNAPGVQI[R-labeled]),
Techniques: Comparison
Journal: JCI insight
Article Title: Metabolism of PLTP, CETP, and LCAT on multiple HDL sizes using the Orbitrap Fusion Lumos.
doi: 10.1172/jci.insight.143526
Figure Lengend Snippet: Figure 3. Compartmental models and kinetics parameters for PLTP and CETP in the larger alpha HDL size fractions. (A) Compartmental model for PLTP and CETP (the average of n = 6 participants). PLTP flux into alpha1 is approximately 45% from the source (0.010 mg/kg/d) and 55% from the smaller alpha2 (0.012 mg/kg/d). Approximately 75% of PLTP on alpha2 is transferred to alpha0 and alpha1 (0.10 and 0.59 pool/d, respectively) while the remaining 25% is removed from the model system (0.20 pool/d). CETP appears on alpha1 and alpha2 via direct secretion. (B) Enrichment curve fits generated from the models in A, participant 1. (C) FCR and production rate (PR) for PLTP and CETP. Bar graphs represent the mean value for n = 6 participants, error bars represent SD, and open circles represent values per participant.
Article Snippet: Each protein was quantified using the following peptide standards: APOA1 (THLAPYSDEL[R-labeled]), APOE (LGPLVEQG[R-labeled]), LCAT (SSGLVSNAPGVQI[R-labeled]),
Techniques: Generated